Nadia OULAHAL (responsable pôle microbiologie)

Mardi 14 janvier 2014 // Pôle Microbiologie

nadia.oulahal@univ-lyon1.fr

Assistant Professor

Address :
BioDyMIA EA n°3733
Université Claude Bernard Lyon 1
IUT - Département Génie Biologique
Technopole Alimentec- rue Henri de Boissieu
01 000 Bourg en Bresse
tel : 00 33 (0)4 74 45 52 52

- Skills

  • food microbiology and food safety with an expertise concerning microbial biofilms and the hygienic status of foods and food-contact surfaces
  • microbial ecology and physiology
  • study and exploitation of bioprotective microorganisms in food systems

- Main Scientific programs

  • « Bioprotective microorganisms and packaged foods preservation » (2010-2013) co-funded by Fonds de Développement de la Recherche du Technopole Alimentec and Région Bourgogne (PARI Agrale N°3 « Matbio Vectorisation ») Partnership : Eaux-Macromolécules-Milieux-Activités (EMMA), AgroSup Dijon, Gpack company, St Martin du Fresne, Actilait (French Technical Institute for Dairy Products) Bourg en Bresse, Centre Technique de la Conservation des Produits Agricoles, Bourg en Bresse and Technopole Alimentec, Bourg en Bresse.
  • « Actiprotex, development of antimicrobial textiles », (2007-2010), competitivity cluster Techtera, coordinator : Institut Français du Textile et de l’Habillement (IFTH), Ecully, partnership : 11 SMEs, 1 company, 4 research laboratories and 2 technical centers.
  • « Purvege : combination of UV-ray and ozone for in situ decontamination of water and air : effect on fruit and vegetables preservation » INTERREG IIIA – France –Switzerland program (2007-2008) Partenership : Ereo SAS (St Genis Pouilly, France), Hautes Etudes Valaisannes, Sion (Switzerland), Université de Genève MANEP-DPMC (Materials with Novel Electric Properties - Département de Physique de la Matière Condensée) (Switzerland), Technopole Alimentec (Bourg en Bresse). Coordinator : LRGIA.
  • « Microbial ecology of cheese ripening wooden shelves. Characterization of the microbial biofilm present on wooden shelves along cheese ripening and determination of their interactions with Listeria monocytogenes. » (2004-2007) Biofibois n°RA 03.27 contract with ACTIA (Association de Coordination Technique de l’Industrie Alimentaire). Partnership : Institut Technique Français des fromages (ITFF now Actilait) (coordinator), Bourg en Bresse, UMR Bioadhésion et Hygiène des Matériau (UBHM) INRA, Massy, AERIAL, Illkirch, Centre Technique du Bois et de l’Ameublement (CTBA), Syndicat Interprofessionnel du Reblochon, Comité National des Appellations d’Origine Laitière, Arilait Recherche, Entremont Alliance company and Metis biotechnologie company.
  • « Coupled methodology to study biofilms on dairy equipments and to estimate contamination risks of dairy products » Contract with the French Ministry for Agriculture and Fisheries (2001-2004), partnership : Unité Sécurité Microbiologique des Aliments - Institut des Sciences et Techniques des Aliments de Bordeaux, Laboratoire de Chimie des Substances Végétales de Bordeaux, Laboratoire de Génie Enzymatique et Biomoléculaire (LGEB) UMR CNRS 5013 (now UMR 5246, ICBMS : Institut de Chimie et Biochimie Moléculaires et Supra-moléculaires) and Laboratoire d’Informatique Graphique – Image et Modélisation (LIGIM) EA 1899 Université Lyon 1, Institut Technique Français des fromages (ITFF) (now Actilait), Bourg en Bresse and Branson company, Rungis. Coordinator : LRGIA.
  • « Development of a method to control cleaning/disinfection procedures efficiency in food industry » (Raliment company, 1996-1998)
  • « Hygienic status of floors in food industry : microbiological assay to control the efficiency of floor cleaning/disinfection procedures. » Alimentec Industries / Alimentec Recherche (Raliment company, 1994)

- Positions/Education

  • Since 2000 : Assistant professor. Consequences of interactions between bacterial cells and solid substrates on cells physiology. Research unit : LRGIA (now BioDyMIA) EA n°3733, IUT – Département Génie Biologique, Université Claude Bernard Lyon 1.
  • 1996 – 1999 : PhD in microbiology - RALIMENT company - Hygiene and Quality Department - Bourg en Bresse
  • 1995 – 1996 : Scientific co-worker – Bellevue Hospital of Saint Etienne – Laboratory of Microbiology

- Scientific production :

Publications :

  • Adt I., Dupas C., Boutrou R., Oulahal N., Noel C., Molle D., Jouvet T., Degraeve P. Beaufort cheese in vitro digestion by gastrointestinal proteases mainly generates caseinphosphopeptides corresponding to alpha caseins fragments. Under revision in International Dairy Journal.
  • Messaoud M., Chadeau E., Brunon C., Ballet T., Rappenne L., Roussel F., Leonard D., Oulahal N., Langlet M.. Photocatalytic generation of silver nanoparticles and application to the antibacterial functionalization of textile supports. Journal of Photochemistry and Photobiology A : Chemistry. In press
  • Brunon C., Bessueille F., Grossiord C., Chadeau E., Oulahal N., Darroux C., Ferreira I., Bourgeois M., Simon F., Rimbault F., Yu C., Leonard D (2010). ToF-SIMS characterization of antimicrobial textiles for the food-processing industry. Surface and Interface Analysis.(www.interscience.wiley.com) DOI 10.1002/sia.3654 in press
  • Chadeau E., Oulahal N., Dubost L., Favergeon F., Degraeve P. (2010). Anti-Listeria innocua activity of silver functionalised textile prepared with plasma technology. Food Control, 21, 505-512.
  • Oulahal N., Adt I., Mariani C., Carnet-Pantiez A., Notz E., Degraeve P.(2009) Examination of wooden shelves used in the ripening of a raw milk smear cheese by FTIR spectroscopy. Food Control, 20, 658-663.
  • Assere A., Oulahal N., Carpentier B. (2008). Comparative evaluation of methods for counting surviving biofilm cells adhering to a Polyvinyl Chloride (PVC) surface exposed to chlorine or drying. Journal in Applied Microbiology, 104 (6), 1692-1702.
  • Oulahal N., Brice W., Martial A., Degraeve P. (2008). Quantitative analysis of survival of Staphylococcus aureus or Listeria innocua on two types of surfaces : polypropylene and stainless steel in contact with three different dairy products. Food Control, 19, 178-185.
  • Oulahal N., Martial-Gros A., Bonneau M., Blum L.J. (2007). Removal of meat biofilms from surfaces by ultrasounds combined with enzymes and/or a chelating agent. Innovative Food Science and Emerging Technology, 8, 192-196.
  • Mariani C., Briandet R.,. Chamba J-.F., Notz E., Carnet-Pantiez A, Eyoug R.N., Oulahal. N. (2007). Biofilm ecology of wooden shelves used in ripening the French, raw milk, smear cheese, Reblochon de Savoie. Journal of Dairy Science, 90(4), 1653-1661.
  • Oulahal N., Martial-Gros A., Bonneau M., Blum L.J. (2004). Combined effect of chelating agents and ultrasound on biofilm removal from stainless surfaces. Application to "Escherichia coli milk" and "Staphylococcus aureus milk" biofilms. Biofilms,1, 65-73.
  • Oulahal N., Martial A., Bonneau M., Blum L.J. (2003). Escherichia coli-milk biofilm removal from stainless steel surface : synergism between ultrasonic waves and enzymes. Biofouling, 19(3), 159-168.
  • Oulahal-Lagsir N., Martial-Gros A ., Bonneau M., Blum L.J. (2000). Ultrasonic methodology coupled to ATP-Bioluminescence for the non invasive detection of fouling in food processing equipment - Validation and application to a dairy factory. Journal in Applied Microbiology, 89, 433-441.
  • Oulahal-Lagsir N., Martial-Gros A ., Boistier-Marquis E ., Blum L.J., Bonneau M. (2000). The development of an ultrasonic apparatus for the non-invasive and repetable removal of fouling in food processing equipment. Letters in Applied Microbiology, 30(1), 47-52.
  • Boistier-Marquis E., Oulahal-Lagsir N., Larpent J-.P. (2000). Methodology for a comparative evaluation of sensitivy to fouling and of cleanability of floors used in the food industry. Biofouling, 14 (4), 279-286.

Proceedings/ book chapters

  • Chadeau E., Brunon C., Degraeve P., Bessueille F., Grossiord C., Ferreira I., Simon F., Leonard D., Oulahal N. (2010). Evaluation of the physiological state of 2 strains after contact with an antimicrobial treated textile. MATBIM 2010. First International Meeting on Material / Bioproduct Interactions. 3-5 March 2010 AgroParisTech, Paris.
  • Lignitto L., Oulahal N., Balzan S., Degraeve P., Dreux N., Segato S. (2008). “Biopeptidi e attività funzionale.” pp 90-102 (capitolo 6) In : “ La produzione e trasformazione del latte nel comprensorio montano vicentino.” (148 pages) coordintaed by Novelli E., Segato S., Balzan S.
  • Oulahal-Lagsir N., Blum L.J., Martial-Gros A. (2005). " Staphylococcus aureus –milk" biofilm removal from stainless steel surfaces : application of ultrasonic waves and enzymes. Récents Progrès en Génie des Procédés. H4 pp 1-7. n°92-2005. ed SFGP : 10ème congrès de la Société Française de Génie des Procédés, Toulouse, september 20-22.
  • Oulahal-Lagsir N., Martial-Gros A., Bonneau M., Blum L.J. (2004). Hygiene testing of stainless steel surfaces : ultrasounds combined to enzymes and a chelating agent to remove meat biofilms. ICEF9 – International Conference on Engineering and Food. Montpellier. Communication n°66
  • Oulahal-Lagsir N., Martial-Gros A., Bonneau M., Blum L.J. (2001). Action combinée d’ultrasons et d’enzymes pour le prélèvement de biofilms en industrie agro-alimentaire. 13èmes Rencontres Scientifiques et Technologiques des Industries Alimentaires. AGORAL Dijon 2001. Editions Tec & Doc, pp329-334 communication n°B-40.
  • Oulahal-Lagsir N., Martial-Gros A., Bonneau M., Blum L.J. (2001). « Optimisation d’un procédé de prélèvement d’un biofilm modèle laitier : action couplée de protéases et d’ultrasons ». GP 499- Th8, 8ème Congrès Français de Génie des Procédés. Nancy., october 17-19 2000.

Patent

  • P16. French patent. Inventors : Lagsir (OULAHAL) N., Boistier E., Grand G. Brevet FR/21.11.97/FR 9714874 : Procédé, dispositif et ensemble autonome de collecte d’impuretés sur une surface. National registering number : 97 14874, publication number : 2 771 314.